Relevance of PKA inhibition to HGFs viability and the production of IL-6, IL-8, and PGE2. HGFs were treated with combinations of LPS (0 and 10 ng/ml) and H-89 (0, 0.1, 1, and 10 μM) for 24 h. (A) Viable cell numbers were measured using WST-8 and are expressed as OD-blank (mean ± S.D., n = 3). (B-D) The concentrations of IL-6, IL-8, and PGE2 were measured by ELISA. The concentrations were adjusted by the viable cell numbers (A) and are expressed as ng or pg per 10,000 cells (mean ± S.D., n = 3). (B) IL-6, (C) IL-8, and (D) PGE2. Open bars, treatment without LPS; closed bars, treatment with 10 ng/ml of LPS. P values were calculated by the pairwise comparison test corrected with the Holm method (6 null hypotheses).
Ara et al. Journal of Negative Results in BioMedicine 2012 11:10 doi:10.1186/1477-5751-11-10