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In vitro studies of the influence of glutamatergic agonists on the Na+,K+-ATPase and K+-p-nitrophenylphosphatase activities in the hippocampus and frontal cortex of rats

Marcos Brandão Contó* and Marco Antonio Campana Venditti

Author Affiliations

Departamento de Psicobiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), Rua Botucatu 862, 1° andar, Vila Clementino, São Paulo, SP, 04023-062, Brazil

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Journal of Negative Results in BioMedicine 2012, 11:12  doi:10.1186/1477-5751-11-12

Published: 10 May 2012



The overstimulation of excitatory glutamatergic neurotransmission and the inhibition of Na+,K+-ATPase enzymatic activity have both been implicated in neurotoxicity and are possibly related to the pathogenesis of epilepsy and neurodegenerative disorders. In the present study, we investigated whether glutamatergic stimulation by the glutamatergic agonists glutamate, α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA), kainate and N-methyl-D-aspartate (NMDA) modulates the Na+,K+-ATPase and the K+-p-nitrophenylphosphatase activities in the crude synaptosomal fraction of the hippocampus and the frontal cortex of rats.


Our results demonstrated that these glutamatergic agonists did not influence the activities of Na+,K+-ATPase or K+-p-nitrophenylphosphatase in the brain structures analyzed. Assays with lower concentrations of ATP to analyze the preferential activity of the Na+,K+-ATPase isoform with high affinity for ATP did not show any influence either.


These findings suggest that under our experimental conditions, the stimulation of glutamatergic receptors does not influence the kinetics of the Na+,K+-ATPase enzyme in the hippocampus and frontal cortex.

Na+,K+-ATPase; K+-p-nitrophenylphosphatase; Glutamate; AMPA; NMDA; Kainate; Frontal cortex; Hippocampus