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Open Access Research

5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells

Wan Kamarul Zaman Wan Safwani1, Suzana Makpol2, Somasundaram Sathapan3 and Kien Hui Chua1*

Author Affiliations

1 Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia

2 Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia

3 Subang Jaya Medical Centre, Selangor, Malaysia

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Journal of Negative Results in BioMedicine 2012, 11:3  doi:10.1186/1477-5751-11-3

Published: 6 January 2012

Abstract

Background

Adipose tissue is a source of multipotent adult stem cells and it has the ability to differentiate into several types of cell lineages such as neuron cells, osteogenic cells and adipogenic cells. Several reports have shown adipose-derived stem cells (ASCs) have the ability to undergo cardiomyogenesis. Studies have shown 5-azacytidine can successfully drive stem cells such as bone marrow derived stem cells to differentiate into cardiomyogenic cells. Therefore, in this study, we investigated the effect 5-azacytidine on the cardiogenic ability of ASCs.

Methods

The cardiogenic potential of ASCs was analysed by studying the morphological changes after induction, the changes in the cardiogenic genes expression i.e. GATA4, MLC-2v, MLC-2a, NKX2.5, β-MHC, α-MHC, Atrial natriuretic peptide (ANP), Connexin 43, Cardiac Troponin C, Cardiac Troponin I and myocyte enhancer factor (MEF2C) and the changes of embryonic stem cells genes expression at P5 and P10 using quantitative PCR.

Results

Our results showed that the induced ASCs did not show significant morphological difference compared to the non-induced ASCs. While quantitative PCR data indicated that most cardiogenic genes and stemness genes expression level decreased after induction at P5 and P10.

Conclusion

5-azacytidine is insufficient for the cardiogenic induction of the ASCs.

Keywords:
5-Azacytidine; Cardiogenic; Ischemia; Adipose; Stem Cells; Differentiation; Heart