Figure 2.

Morphology of ASCs after undergoing cardiogenic differentiation using 5-azacytidine for 24 hours and maintained with basal medium for 3 weeks. A) The ASCs after 24 hours of induction. B) The induced ASCs gradually increased in size after 1 week of induction. C) The induced ASCs lengthened and formed stick-like morphology at 2 weeks of induction. D) The induced ASCs formed a myotube-like structure after 2 weeks of induction. E) The ASCs formed a ball-like appearance after 2 weeks of induction but there was no spontaneous beating even after 4 weeks of induction. (Magnification 200×)