Table 1

Description of cardiogenic gene primers used in quantitative PCR

Gene

Accession No

Primer 5' 3'

PCR product size

(bp)


GATA-4

NM_002052

R 5'-CTCCTACTCCAGCCCCTACC-3'

207

F 5'-GTGGACATAGCCCCACAGTT-3'

MLC-2a

NM_021223

R 5'-GCAGACCTGAGGGAGACCTAC-3'

135

F 5'-ATTGAGCTTCTCCCCAAAGAG-3'

MLC-2v

NM_000432

R 5'-ACAGGGATGGCTTCATTGAC-3'

192

F 5'-ATGCGTTGAGAATGGTTTCC-3'

Nkx2.5

NM_004387

R 5'-GAGAAGACAGAGGCGGACAA-3'

187

F 5'-AGATCTTGACCTGCGTGGAC-3'

β-MHC

NM_000257

R 5'-TGATCTGGAGCTGACACTGG-3'

196

F 5'-CAGGGTGTTGACCTTGTCCT-3'

α-MHC

NM_002471

R 5'-CCACCCAAGTTCGACAAGAT-3'

127

F 5'-CACAGAAGAGGCCCGAGTAG-3'

ANP

NM_000906

R 5'-GCATTGAGCTGACACGAAAA-3'

219

F 5'-CCTTGACGATGTCATTGGTG-3'

Connexin 43

NM_000165

R 5'-GGACATGCACTTGAAGCAGA-3'

103

F 5'-GATGATGTAGGTTCGAAGCA-3'

TnIc

NM_000363

R 5'-CTCCAACTACCGCGCTTATG-3'

116

F 5'-CCAGCTCTTGCTTTGCAATC-3'

TnTc

NM_000364

R 5'-CATGGAGAAGGACCTGAATGA-3'

108

F 5'-CGTCTCTCGATCCTGTCTTTG-3'

MEF2C

NM_002397

R 5'-CAGTCATTGGCTACCCCAGT-3'

221

F 5'-GCAGATGGTGGCATGTTATG-3'

Desmin

NM_001927

R 5'-GCCTCATCAGGGAATCGTTA-3'

129

L 5'-AGGCAGCCAACAAGAACAAC-3'

α-Actin

NM_005159

R 5'-AGGGGCTGGAAGAGTGTCTCA-3'

136

L 5' -GCCCTGGATTTTGAGAATGA-3'


Wan Safwani et al. Journal of Negative Results in BioMedicine 2012 11:3   doi:10.1186/1477-5751-11-3

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