Table 2 |
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|
Description of stemness gene primers used in quantitative PCR |
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|
Gene |
Accession No |
Primer 5' 3' |
PCR product size (bp) |
|
|
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|
GAPDH |
NM_002046 |
R 5'-GGAGGAGTGGGTGTCGCTGT-3' |
217 |
|
F 5'-TCCCTGAGCTGAACGGGAAG-3' |
|||
|
Sox2 |
NM_003106.2 |
R 5'GGTAGTGCTGGGACATGTGAA-3' |
132 |
|
F 5'-TTACCTCTTCCTCCCACTCCA-3' |
|||
|
FGF4 |
NM_002007 |
R 5'-GGTTCCCCTTCTTGGTCTTC-3' |
118 |
|
F 5'-GATGAGTGCACGTTCAAGGA-3' |
|||
|
Nanog3 |
NM_024865.2 |
R 5'-TGTTTGCCTTTGGGACTGGT-3' |
153 |
|
F 5'CTGTGATTTGTGGGCCTGAA-3' |
|||
|
Oct-4 |
NM_002701 |
R 5'-GAAGTGAGGGCTCCCATAGC-3' |
180 |
|
F 5'-AAGGATGTGGTCCGAGTGTG-3' |
|||
|
|
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|
Wan Safwani et al. Journal of Negative Results in BioMedicine 2012 11:3 doi:10.1186/1477-5751-11-3 |
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