Table 4

Primers for FKBP12 and FKBP12.6 amplification of coding exons and flanking region sequences.

Gene

Exon

Primer

Amplicon

FKBP12

1F

5'- TCTCGGGGCTTCTGGGCTTC -3'

269

1R

5'- GCGCCACTACTCACCGTCTC -3'

2F

5'- CATGCCCGTCTCTGTCTCCTCA -3'

242

2R

5'- AAGCCCCAGGCTGCCCCTGA -3'

3F

5'- TGCTGCTGGTTTCTGACTTGT -3'

299

3R

5'- TGGCATGAGGGTGAGACTTTTC -3'

4F

5'- TTTCTTACAGAGACAGTTGGGG -3'

253

4R

5'- GCCCTTCAGTATTCCATTTCCT -3'

FKBP12.6

1F

5'- GAATGGATGGATGGAGGATG -3'

527

1R

5'- TCCTAGACCCCCAAGAATCC -3'

2F

5'-CCAGAGAGGTGACGTGA-3'

390

2R

5'-TAGACTGGCTGGGAGGA-3'

3F

5'-GGGAGTTTACTCATGACCA-3'

368

3R

5'-CAGGGACACAAAAGAGGTA-3'

4F

5'-CAGTCACATCTCTGCTGA-3'

510

4R

5'-CGCATGAACACTGACGAA-3'

Biagi et al. Journal of Negative Results in BioMedicine 2012 11:4   doi:10.1186/1477-5751-11-4