Figure 1.

Granulysin-GFP fusion protein is expressed and secreted but doesn't colocalize with perforin. a) Immunoblot analysis using anti-GFP sera was performed on cell lysate and cell media supernatant samples of YT cells expressing granulysin-GFP fusion protein (YT.granGFP) and native GFP (YT.GFP). b) Confocal immunofluorescence staining for granulysin (green) and perforin (red) was performed for YT expressing native granulysin (YT.granulysin) and granulysin-GFP fusion protein (YT.granGFP).