Figure 4.

Treatment of HeLa cervical carcinoma cells with CP-31398 leads to p53 dependent induction of the luciferase reporter. HeLa cells were transfected with a p53 responsive reporter gene (WWP-luc) and a reference β-galactosidase plasmid (CMV-lacZ) for normalization. Control cells were transfected with CMV-lacZ alone. The cells were subsequently incubated with CP-31398 (0–10 μg/ml) and relative luciferase activities were determined. Cells were treated for 16 hours.