Figure 6.

Viability index in CD34-positive mononuclear cell cultures. Cells were treated with transfection reagents for 2 h, followed by culture for 4 h, 8, or 24 h. CD34-positive cells were grown on collagen-coated plates and after transfection, cells were stained with Calcein AM and EthD. Fluorescence of the dyes was measured using the microplate reader and appropriate filter sets. BL, baseline with no additions; Ad, adenoviral infection of GFP; T1-T8, transfection reagents as shown in Tables 1 and 2. ANOVA: p < 0,001