Table 2 |
||||
|
Transfection efficiency (% of live cells) in CD34-positive mononuclear cell cultures |
||||
|
1 h |
24 h |
48 h |
5 d |
|
|
|
||||
|
Baseline |
0 |
0,13 ± 0,031 |
0,47 ± 0,08 |
0 |
|
Adenovirus |
2,7 ± 0,12 |
13,1 ± 1,4 |
22,6 ± 3,4 |
4,2 ± 0,96 |
|
T1: Metafectene |
0 |
0,12 ± 0,042 |
0,23 ± 0,053 |
0 |
|
T2: Lipofectamine plus |
0 |
0,24 ± 0,060 |
0,30 ± 0,11 |
0 |
|
T3: Tfx-50 |
0 |
0,10 ± 0,037 |
0,41 ± 0,091 |
0 |
|
T4: FuGene6 |
0 |
0,23 ± 0,071 |
0,22 ± 0,13 |
0 |
|
T5: DOTAP |
0 |
0 |
0,56 ± 0,064 |
0 |
|
T6: DOSPER |
0 |
0,16 ± 0,046 |
0,28 ± 0,13 |
0 |
|
T7: JetPei |
0 |
0,20 ± 0,050 |
0,50 ± 0,15 |
0 |
|
T8: DuoFect |
0 |
0 |
0,12 ± 0,056 |
0 |
|
|
||||
|
GFP-expressing and negative osteoclasts were counted using fluorescence microscopy and phase optics, and transfection efficiencies were counted. ANOVA: p = 2,3 × 10-11, n = 5. |
||||
|
Laitala-Leinonen Journal of Negative Results in BioMedicine 2005 4:5 doi:10.1186/1477-5751-4-5 |
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