www.jnrbm.com

Table 1
*Description of PLIN* SNPs, primers, PCR conditions and restriction enzymes.**
SNP	Primers	PCR (bp)	MgCl₂(mM)	Ann. temp.	RE	Size (bp) for the wt allele	Size (bp) for the mut allele	MAF (%)

rs4578621	F : CAAGCTGGGTGCACTGGC R : GAGAAATAGAGGAATTAACC	185	0.9	58	MnlI	33+152	33+62+90	7.6
rs6496589	F : CTGCCAACACTCG AGCTG R : ACCTGACTCTTCCTTGTCT	110	1	60	PstI	72+28	no cut	0.6
rs894160	F1 : GCTGAGACTGAGTCACATGC R1 : GCTGAGACTGAGTCACATGC	403	0.5	60	-	-	-	-
	F2 : CTGTTTGTGGGGCTCCCTCG R2 : CCTCCCAGATCTTTTAAGAG	126 (nested)	0.5	52	XhoI	126	20+106	30.0
rs8179070	F : CAGCTATCTGCTGCCATC R : CTCACTGAACTTGTTCTCC	219	0.5	58	MspI	75+144	219	ND
rs3743373	F : CAGCTATCTGCTGCCATC R : CTCACTGAACTTGTTCTCC	219	0.5	58	BseRI	96+49+45+21+5+3	96+94+21+ 5+3	ND
rs8179071	F : GTAGCAGCCCTGCCAGGCC R : CCGGCACGTAATGCACCAC	248	0.5	69	BstXI	66+182	66+129+53	0.6
rs8179072	F : GTAGCAGCCCTGCCAGGCC R : CCGGCACGTAATGCACCAC	248	0.5	69	Hpy188III	248	80+168	ND

F : forward, R : reverse, wt allele : wild-type (common) allele, mut allele : mutant (rare) allele, Ann. : annealing, temp. : temperature, RE: restriction enzyme, MAF: minor allele frequency. The SNP allele frequencies were determined in 90 individuals.
Meirhaeghe et al. Journal of Negative Results in BioMedicine 2006 5:10 doi:10.1186/1477-5751-5-10

Table 1