Figure 3.

Western blot analyses of VEGF-induced signal transduction in differentiated PC12 cells after NGF withdrawal. Lysates of control cells maintained in the presence of NGF were loaded in lane 1. NGF-deprived PC12 cells (lane 2) treated with VEGF₁₆₅ (lanes 3, 4) or NGF (lanes 5, 6) demonstrated that VEGF₁₆₅ induced transient activation of ERK1/2 and Akt after 7 min. In contrast, NGF produced a stronger and persistent phosphorylation of ERK1/2 and Akt than VEGF₁₆₅.