Figure 3.

SRY Amplification using Real Time PCR. A. Amplification curves of all samples. The negative control did not have any amplification. Some DNA samples from group 1 and group 2 were not included in the analysis due to low quality of the DNA extracted. B. Quantitative Analysis Report. Amplification after cycle 41 was taken as background DNA and no copies where reported in the Rotor-Gene 6 software. Ct = Cycle Threshold, Calc Conc = Calculated Concentration, T/T = Total Copies in the Thyroid Gland (Assuming both thyroid lobes were the same size).