Figure 2.

NT2/D1 cells in IAM medium. Cell culture images of NT2/D1 cells after 20 days growing in control, IAM and IAM+BMP2 medium. All the non fluorescent and fluorescent images are 100× magnified and analyzed in a CK2 and BX50 Olympus microscope, respectively. Ultrastructural images have a scale bar in the bottom right corner. NT2/D1 cells formed many embryonic body-like structures (white arrows), surrounded by an epithelioid squamous layer of adherent flattened cells. Desmosomes can be seen between adjacent cells (TEM black arrow). To see non fluorescent and fluorescent images of days 1, 5, 10 and 30, please [see Additional file 4].