Figure 4.

HPTLC of Ganglioside Distribution in Hexb+/- and Hexb-/- Mice. HPTLC of two Hexb+/- and two Hexb-/- samples show the ganglioside distribution of sciatic nerve tissue. For each sample, gangliosides having approximately 1.3 μg of sialic acid were spotted on the HPTLC plates. The plates were developed by a single ascending run with chloroform:methanol:dH₂O (55:45:10, v:v) containing 0.02% CaCl₂·2H₂O. GM2 is present in the Hexb-/- lanes (arrows) and undetectable in the Hexb+/- lanes. The identity of the GM2 band was confirmed using an external standard (Hexb-/- brain tissue, neural tube).