Figure 1.

Genotyping of MMP2 -735 C/T SNP by melting temperature analysis. (A) The top panels depict the derivative melting curve plots obtained for (from left to right): MMP2 -735CC, MMP2 -735CT, MMP2 -735TT, and an unexpected profile (UKN) showing peaks between 62 and 66°C (B) The bottom panel depicts the wild type (left) and a new variant (right) at position -729 near the target SNP (white arrow). Reverse sequencing revealed a novel mutation corresponding to a G to A change at position -729 in the sense strand (black arrow).