Table 1

Polymorphisms typed in the EDN, ECP, EPO and MBP genes.
Gene
 Rs number
 Location
 Amino acid
 Primer
 Annealing temperature
 Restriction enzyme
EDN
 rs2013109
 Intron 1
 -
 F: GGGTAAGTCAACGATCCCCAG
R: GGTCTTGGTTATGACACACACTGTAGT
 69°C
 HpyF10VI
EDN
 rs10132319
 Intron 1
 -
 F: GGGTAAGTCAACGATCCCCAG
R: GGTCTTGGTTATGACACACACTGTAGT
 65°C
 AciI
ECP
 rs17792481
 Intron 1
 -
 F: TGAGGGAGAGGTGAGCTGAAGT
R: TGATGTGCTGGATGGCAAAC
 58°C
 MboI
ECP
 rs2073342
 Exon 2
 T124R
 F: TACGCTGCCCTCATAACAGAACT
R: GAACTGGAACCACAGGATACCG
 58°C
 PstI
ECP
 rs2233860
 3' UTR
 -
 F: GTATGCAGACAGACCAGGAAGGA
R: TTGGCAGATGAGTGATGATGAGTA
 61°C
 RsaI
EPO
 rs11652709
 Exon 4
 Q122H
 F: CTACCCTGGCCTGGAGTAGAAG
R: CACGCACTTGTTGTTGCACC
 64°C
 HpyF10VI
EPO
 rs3785496
 Intron 6
 -
 F: ACTGGTTGTCACTTCCCCTCTC
R: CAAAACCTTGCTTAAAACTCCCTT
 58°C
 MseI
MBP
 rs490358
 Exon 2
 -
 F: GGAAAAAACAGAGAAAGAAAGACTGAA
R: CACTCCCACGGTCCCCTAAT
 58°C
 BslI
MBP
 rs3741098
 Intron 3
 -
 F: TGGTGGACAAAAACCTTACGTGTC
R: ATGCTGGGGGCATATCCGA
 58°C
 BsaHI

Parwez et al. Journal of Negative Results in BioMedicine 2008 7:9   doi:10.1186/1477-5751-7-9