Your browser version may not work well with NCBI's Web applications. More information here...
1: Science. 1992 Jul 10;257(5067):235-7.
Related Articles, Links
Click here to read
Modulation of DNA binding specificity by alternative splicing of the Wilms tumor wt1 gene transcript.

Bickmore WA, Oghene K, Little MH, Seawright A, van Heyningen V, Hastie ND.

Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.

The technique of whole-genome polymerase chain reaction was used to study the DNA binding properties of the product of the wt1 gene. The zinc finger region of this gene is alternatively spliced such that the major transcript encodes a protein with three extra amino acids between the third and fourth fingers. The minor form of the protein binds specifically to DNA. It is now shown that the major form of wt1 messenger RNA encodes a protein that binds to DNA with a specificity that differs from that of the minor form. Therefore, alternative splicing within the DNA binding domain of a transcription factor can generate proteins with distinct DNA binding specificities and probably different physiological targets.

Publication Types:
PMID: 1321494 [PubMed - indexed for MEDLINE]